Neutrophils of red seabream Pagrus major contain stratified granules with eosinophilic core (layer 0, L0) and chromophobic surrounding area (mantle； layer 1, L1) of L0. After staining with peroxidase (PO)-detection methods (PO is localized in L1), dark brown visible light (long-wavelength light) radiate from L0 (PO-negative), and is recognized to ‘spot’ under light microscope. The light will radiate from inner structure of L0 (inner core, L0-0).