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URIhttp://ypir.lib.yamaguchi-u.ac.jp/fu/metadata/82
タイトルシベリアチョウザメの顆粒球の形態学的および細胞科学的特徴
タイトルヨミシベリアチョウザメ ノ カリュウキュウ ノ ケイタイガクテキ オヨビ サイボウカガクテキトクチョウ
タイトル別表記Morphological and Cytochemical Characteristics of Granulocytes from Siberian Sturgeon Acipenser baerii
作成者近藤, 昌和
酒井, 麻帆
安本, 信哉
高橋, 幸則
作成者ヨミコンドウ, マサカズ
サカイ, マホ
ヤスモト, シンヤ
タカハシ, ユキノリ
作成者別表記Kondo, Masakazu
Sakai, Maho
Yasumoto, Shinya
Takahashi, Yukinori
著者キーワードSiberian sturgeon
Acipenser baerii
granulocyte
morphology
cytochemistry
資料タイプtext
ファイル形式application/pdf
内容記述(抄録等)Three types of granulocytes, neutrophil, eosinophil and small eosinophilic granulocyte, were observed in peripheral blood of Siberian sturgeon Acipenser baerii. The neutrophil had three types of granules, eosinophilic granule (αG; orange), chromophobic granule (βG) and basophilic granule (γG; light blue) in the cytoplasm. These granules were round or oval, but the maximum diameter was different each other (αG, ≦0.3 μm; βG, ≦1.5 μm; γG, ≦0.4 μm). The nucleus of the neutrophil showed polymorphism, round, oval, kidney and multi-lobes (to five lobes). Alkaline phosphatase (AlP), acid phosphatase (AcP), β-glucronidase (β-Glu) and some esterases were detected as positive granules. Peroxidase (PO) activity was not observed in the neutrophil. Eosinophil had polymorphic nucleus and two types of eosinophilic granules (EG), large EG (LEG) and small EG (SEG). Both granules were round or oval and the diameter of LEG (≦1.1 μm) was larger than SEG (≦0.4 μm). The former was stained orange, the latter showed brick color. In eosinophil, AcP, β-Glu and some esterases were positive, but AlP and PO were negative. Small eosinophilic granulocyte was small sized cell with condensed nucleus and some eosinophilic granules. The cell was round to oval, and had high nuclear to cytoplasmic ratio. The morphology and staining characteristics of the granules were similar to LEG of eosinophil. No enzyme activities were detected in the granulocyte. Phagocytosis against zymosan particles was observed in only neutrophil.
出版者水産大学校
出版者ヨミスイサン ダイガッコウ
Nii資料タイプ紀要論文
本文言語jpn
ISSN0370-9361
掲載誌名水産大学校研究報告
63
4
開始ページ262
終了ページ270
発行日2015-03
著者版/出版社版出版社版
リポジトリIDNF0063405
ファイル63-4-262-270.pdf ( 1.9MB ) 公開日 2016-09-27
査読の有無査読あり
地域区分水産大学校