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Neutrophil granules of red seabream Pagrus major infected with gliding bacterium Tenacibaculum maritimum

水産大学校研究報告 Volume 69 Issue 3 Page 69-73
published_at 2021-03
69-3-69-73.pdf
[fulltext] 654 KB
Title
滑走細菌Tenacibaculum maritimum に感染させたマダイの好中球顆粒
Neutrophil granules of red seabream Pagrus major infected with gliding bacterium Tenacibaculum maritimum
Creators Kondo Masakazu
Creators Watanabe Riho
Creators Tachibanagi Keito
Creators Kimura Takehiro
Creators Yasumoto Shinya
Source Identifiers
Creator Keywords
granule neutrophil red seabream Pagrus major tenacibaculosis Tenacibaculum maritimum
Three types of granules, two extraordinary types and one inducible type, were observed in the neutrophils of red seabream Pagrus major infected with Tenacibaculum maritimum. Both extraordinary granule types (eoG1^{Tm}, eoG2^{Tm}) had similar morphologies but different cytochemical characteristics to those of ordinary granules (oG1^N, oG2^N) from non-infected fish. The eoG1^{Tm} showed chromophobic, simple morphology (without stratified structure), peroxidase positive and lack of lysozomal enzymes. The eoG2^{Tm} was stratified granule with three-layer structure [inner eosinophilic layer (L0-0), middle chromophobic layer (L0-1) and outer chromophobic layer (L1)]. Acid phosphatase, ꞵ-glucuronidase (ꞵ-Glu), α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in L0-0, but α-naphtyl butylate esterase was in L0 (L0-0 and L0-1). Peroxidase (PO) were localized in L1. Both extraordinary types were Sudan black B (SBB) negative. Spot formation, a curious phenomenon appeared in PO-stained oG2^N (positive L1 and negative L0), was not observed in eoG2^{Tm}. Inducible granule (iG^{Tm}) was consisted of two chromophobic layers (inner L0 and outer L1). The L0 of iG^{Tm} showed positive reaction to PO.
Languages jpn
Resource Type departmental bulletin paper
Publishers 水産大学校
Date Issued 2021-03
File Version Version of Record
Access Rights open access
Relations
[ISSN]0370-9361